Journal: Journal of ethnopharmacology

Article Title: Anemarrhena asphodeloides Bunge and Phellodendri Chinensis Cortex inhibits the PTGS2/EP2/cAMP/Epac1 signaling pathway to reduce microglial M1 polarization, thereby blocking chronic stress-induced depression-like behavior.

doi: 10.1016/j.jep.2025.119792

Figure Lengend Snippet: Fig. 8. Neomangiferin alleviates the release of proinflammatory factors in BV2 cells by inhibiting PTGS2/EP2/cAMP/Epac1 signals. (A) representative immunofluorescence staining of EP2 and Epac1, and (B) fluorescence area (n = 3). (C–D) Protein expression changes of EP2 and Epac1 (n = 3). The contents of PGE2 and cAMP in BV2 cells were determined by E-F Elisa kit (n = 3). (G) Representative images of zebrafish embryos in Neomangiferin experiment (n = 10). Data are expressed as mean±SD. *** means P < 0.001,** means P < 0.01,* means P < 0.05, ns means P > 0.05.

Article Snippet: Seal with 5 % skim milk powder at room temperature for 2 h. Primary antibody β-actin (rabbit, 1:5000; bs-00R,Bioss, China), GAPDH(rabbit, 1:10,000; 10494- 1-AP,Proteintech, China), HSP90(rabbit, 1:5000; 13171-1-AP,Proteintech, China), Arg-1 (rabbit, 1:1000; 16001-AP, Proteintech, China), iNOS (rabbit, 1:1000; ab283655, Abcam, UK), PTGS2 (mouse, 1:400; 66351-1-Ig, Proteintech, China), EP2 (rabbit, 1:1000; HA721380, HUABIO, China), EPAC1 (rabbit, 1:1000; A02483-3, Boster, China), incubated overnight at 4 ◦C.

Techniques: Immunofluorescence, Staining, Fluorescence, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: VLPs characterization. MSP-8 ( A ), MSP-9 ( B ) and RAP1 ( C ) VLP morphologies were visualized under TEM. MSP-8 ( D ), MSP-9 ( E ) and RAP1 ( F ) VLPs were loaded (8, 40 µg) for SDS-PAGE, and bands corresponding to each antigen were observed using either the polyclonal P. berghei antibody or the monoclonal M1 antibody.

Article Snippet: Codon-optimized RAP1 was synthesized by GenScript.

Techniques: SDS Page

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: Experimental schedule. Mice were immunized twice with the combined VLP vaccines expressing MSP-8, MSP-9, and RAP1 of P. berghei at 4 weeks intervals, and blood samples were drawn 3 weeks after each immunization. Challenge infection was performed 4 weeks after boost immunization and half of the mice in each group were sacrificed at 1 week post-challenge.

Article Snippet: Codon-optimized RAP1 was synthesized by GenScript.

Techniques: Vaccines, Expressing, Infection

Journal: Vaccines

Article Title: Protection and Alleviated Inflammation Induced by Virus-like Particle Vaccines Containing Plasmodium berghei MSP-8, MSP-9 and RAP1

doi: 10.3390/vaccines10020203

Figure Lengend Snippet: IgG antibody response. Mice were intramuscularly immunized twice with the mixture of MSP-8, MSP-9 and RAP1 VLPs. Sera ( n = 6) were collected at regular intervals after each immunization with the VLPs. VLPs immunization induced IgG antibodies reacted with P. berghei Ag ( A ) and VLPs ( B ) upon prime and boost compared to naïve sera (** P < 0.01, *** P < 0.001).

Article Snippet: Codon-optimized RAP1 was synthesized by GenScript.

Techniques:

Journal: Experimental & Molecular Medicine

Article Title: MiR-1224-5p modulates osteogenesis by coordinating osteoblast/osteoclast differentiation via the Rap1 signaling target ADCY2

doi: 10.1038/s12276-022-00799-9

Figure Lengend Snippet: Antibody listing.

Article Snippet: Mouse anti-Rap1 , Affinity Biosciences Cat# DF8598, RRID:AB_2841802.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Cholesterol-Rich Microdomains Contribute to PAR1 Signaling in Platelets Despite a Weak Localization of the Receptor in These Microdomains

doi: 10.3390/ijms21218065

Figure Lengend Snippet: Weak colocalization of PAR1 and cholesterol-rich microdomains in human platelets. ( A ) Double staining of PAR1 (green) and cholesterol-rich microdomains (red) showing no significant colocalization of PAR1 in cholesterol-rich microdomains in resting (Rest) platelets and a weak but significant increase of colocalization in TRAP-stimulated platelets. Images shown are representative of five independent experiments (15 images were analyzed). The insets show an enlargement of the indicated region (scale bar = 5 µm). ( B ) Statistical tests for measures of colocalization between PAR1 and cholesterol-rich microdomains confirm a weak but significant increase in TRAP-activated platelets. This colocalization remained low as the coefficient of colocalization measured by different tests including Pearson’s, Van Steensel’s, and Costes’ was lower than 0.5. * p < 0.05, ** p < 0.005 (according to a student t test), n = 5.

Article Snippet: Mouse monoclonal anti-PAR1 (61152, BD Biosciences, San Jose, CA, USA) and monoclonal anti-flotillin-1 (610821, BD Biosciences, San Jose, CA, USA) were used for immunoblotting analysis.

Techniques: Double Staining

Journal: International Journal of Molecular Sciences

Article Title: Cholesterol-Rich Microdomains Contribute to PAR1 Signaling in Platelets Despite a Weak Localization of the Receptor in These Microdomains

doi: 10.3390/ijms21218065

Figure Lengend Snippet: Assessment of colocalization of PAR1 and cholesterol-rich microdomains by a biochemical approach. ( A ) Flotillin-1, a marker of cholesterol–rich microdomains, was mainly detected in fractions 2–4 in all experimental groups; ( B ) PAR1 was mainly present in fractions 10–12, which did not contain cholesterol-rich microdomains; ( C ) Quantitative dot blot of flotillin-1 and PAR1 in experimental groups compared to control.

Article Snippet: Mouse monoclonal anti-PAR1 (61152, BD Biosciences, San Jose, CA, USA) and monoclonal anti-flotillin-1 (610821, BD Biosciences, San Jose, CA, USA) were used for immunoblotting analysis.

Techniques: Marker, Quantitative Dot Blot

Journal: International Journal of Molecular Sciences

Article Title: Cholesterol-Rich Microdomains Contribute to PAR1 Signaling in Platelets Despite a Weak Localization of the Receptor in These Microdomains

doi: 10.3390/ijms21218065

Figure Lengend Snippet: Thrombin signaling through proteinase-activated receptor participating proteins. ( A ) Proteins which showed significant difference in number of spectra in experimental groups. * p < 0.05 according to one-way ANOVA test. ( B ) Network analysis of proteins detected in thrombin signaling through proteinase-activated receptors. GBB: guanine nucleotide-binding protein GI/GS/GT subunit beta; GNA: guanine nucleotide-binding protein subunit alpha; GNAQ: guanine nucleotide-binding protein G(q) subunit alpha; GNB: guanine nucleotide-binding protein subunit beta; Src: proto-oncogene tyrosine-protein kinase; PAR: protease activated receptor; F2R (PAR1): coagulation factor II thrombin receptor; F2RL3 (PAR4): F2R like trypsin receptor 3.

Article Snippet: Mouse monoclonal anti-PAR1 (61152, BD Biosciences, San Jose, CA, USA) and monoclonal anti-flotillin-1 (610821, BD Biosciences, San Jose, CA, USA) were used for immunoblotting analysis.

Techniques: Binding Assay, Coagulation

Journal: eLife

Article Title: Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy

doi: 10.7554/eLife.88088

Figure Lengend Snippet: Summary of all antibodies and stains used in this study.

Article Snippet: Anti-RAP1 (2.29) , Mouse , European Malaria Reagent Repository , , , , 1:500 , , , .

Techniques: Concentration Assay, Generated, Staining